Subject:  Copy of PUBMED (EMF research) Article (Bormann).. 
Date:     Sat, 1 Apr 2000 091906 -0600 (CST)
From:     "Roy L. Beavers" 
To:       emfguru 
--------------------------------------------------


.......Yes, this is interesting......  Hope we may hear from some
of our researchers on this.....  Are these results ACTUALLY as
recent as they appear to be???

Specifically, is not this experiment **very similar to** (a replication)
of the Reba Goodman and Martin Blank work of two or three years ago???

Cheerio......(Thank you, Dr. Bormann...)

Roy Beavers (EMFguru)
roy@emfguru.com

.....It is better to light a single candle than to curse the darkness.....
                    NEW!!! Website... http://emfguru.com
...................People are more important than profits.................
                          
             DO YOU KNOW OF OTHERS WHO SHOULD BE ON THIS LIST???

---------- Forwarded message ----------
Date: Thu, 30 Mar 2000 20:39:15 -0800
From: "Carolyn Bormann, N.D. ,C.P." 
To: "Roy L. Beavers" 
Subject: Copy of PUBMED Article I thought you'd find interesting...

Dear Roy:

Thanks for the welcome.  The Travolta case I was discussing was the
movie released last Fall "Class Action" with John Travolta.  Rent it at
the video store.  The movie sounds like it might have been based on the
Love Canal thing, but I'm not sure.  Environmental poisioning is the
issue...and he loses everything fighting the "big bad chemical
companies".  

Oh well...we keep plugging along as God enables...

Dr. Bormann   [......See below.....]
******************

Cardiovasc Res 2000 Mar;45(4):1054-64

Elf-pulsed magnetic fields modulate opioid peptide gene expression in
myocardial cells.
Ventura C, Maioli M, Pintus G, Gottardi G, Bersani F Department of
Biomedical Sciences, University of Sassari, Italy.
chim_med@ssmain.uniss.it

[Medline record in process]

OBJECTIVES: Magnetic fields have been shown to affect cell proliferation
and growth factor expression in cultured cells. Although the activation of
endorphin systems is a recurring motif among the biological events
elicited by magnetic fields, compelling evidence indicating that
magnetic fields may modulate opioid gene expression is still lacking. We
therefore investigated whether extremely low frequency (ELF) pulsed
magnetic fields (PMF) may affect opioid peptide gene expression and the
signaling pathways controlling opioid peptide gene transcription in the
adult ventricular myocyte, a cell type behaving both as a target and as a
source for opioid peptides.

METHODS: Prodynorphin gene expression was investigated in adult rat
myocytes exposed to pulsed-magneti-fields (PMF) by the aid of RNase
protection and nuclear run-off transcription assays. In PMF-exposed
nuclei, nuclear protein kinase C (PKC) activity was followed by measuring
the phosphorylation rate of the acrylodan-labeled MARCKS peptide. The
effect of PMF on the subcellular distribution of different PKC isozymes
was assessed by immunoblotting. A radioimmunoassay procedure coupled to
reversed-phase high performance liquid chromatography was used to monitor
the expression of dynorphin B.

RESULTS: Here, we show that PMF enhanced myocardial opioid gene expression
and that a direct exposure of isolated myocyte nuclei to PMF
markedly enhanced prodynorphin gene transcription, as in the intact
cell. The PMF action was mediated by nuclear PKC activation but occurred
independently from changes in PKC isozyme expression and enzyme
translocation. PMF also led to a marked increase in the synthesis and
secretion of dynorphin B.

CONCLUSIONS: The present findings demonstrate that an opioid gene is
activated by myocyte exposure to PMF and that the cell nucleus and nuclear
embedded PKC are a crucial target for the PMF action. Due to the
wide ranging importance of opioid peptides in myocardial cell
homeostasis, the current data may suggest consideration for potential
biological effects of PMF in the cardiovascular system.
PMID: 10728432, UI: 20192676
http://www.ncbi.nlm.nih.gov:80/entrez/query.fcgi?CMD=Save&DB=PubMed




Archive provided courtesy of WaveGuide, http://www.wave-guide.org
Reprinted with permission of Roy Beavers, http://www.emfguru.com