Subject: Important new research (Segerback).
Date: Tue, 12 Sep 2000 050325 -0500
From: Roy Beavers
To: guru
--------------------------------------------------
.........From EMF-L......
Per Segerback has forwarded some very interesting recent research
results. I find the gene-expression results particularly interesting.
My recollection is that a Japanese researcher also reported gene-
expression results at the recent BEMS meeting in Munich.....
I had first questioned Per about the publication dates......guru.....
-------- Original Message --------
Subject: Details on MW + LF
Date: Tue, 12 Sep 2000 10:08:59 +0200
From: Per SegerbSck
To: "Roy Beavers"
*************
Roy,
This is "normal" for Medline. I have seen it for years. I think it
depends on that they receive the information (abstracts, etc) long
before the paper copy shows up.
Best Regards
Per S
----- Original Message -----
From: "Roy Beavers"
To: "Per Segerbäck"
Cc: "guru"
Sent: Saturday, September 09, 2000 1:29 AM
Subject: Re: MW + LF
Per:
Those publication dates don't look right??? How is it possible for
1 Dec 2000 to have already been published...... When were these
actually done?? Have they been in hiding someplace....guru......
----------------------------------------------------------------------
Per Segerbäck wrote:
All,
Two interesting studies have been published recently. The first
indicates that also 1.8GHz GSM opens up the Blood Brain Barrier,
the second shows changes in human cell transcription due to 50Hz
magnetic field exposure.
***************
Bioelectromagnetics 2000 Jul;21(5):338-45
Electromagnetic fields (1.8 GHz) increase the permeability to sucrose
of the blood-brain barrier in vitro.
Schirmacher A, Winters S, Fischer S, Goeke J, Galla H, Kullnick U,
Ringelstein EB, Stogbauer F
Klinik und Poliklinik fur Neurologie, Universitat Munster, Germany.
[Medline record in process]
We report an investigation on the influence of high frequency
electromagnetic fields (EMF) on the permeability of an in vitro model
of the blood-brain barrier (BBB). Our model was a co-culture consisting
of rat astrocytes and porcine brain capillary endothelial cells (BCEC).
Samples were characterized morphologically by scanning electron
microscopy and immunocytochemistry. The BBB phenotype of the BCEC was
shown by the presence of zona occludens protein (ZO-1) as a marker for
tight junctions and the close contact of the cells together with the
absence of intercellular clefts. Permeability measurements using
(14)C-sucrose indicated a physiological tightness which correlated with
the morphological findings and verified the usefulness of our in vitro
model.
Samples were exposed to EMF conforming to the GSM1800-standard used in
mobile telephones (1.8 GHz). The permeability of the samples was monitored
over four days and compared with results of samples that were cultured
identically but not exposed to EMF.
Exposure to EMF increased permeability for (14)C-sucrose significantly
compared to unexposed samples. The underlying pathophysiological
mechanism remains to be investigated. Copyright 2000 Wiley-Liss, Inc.
PMID: 10899769, UI: 20360103
*****************
J Cell Biochem 2000 Dec 1;79(3):460-470
The effect of 50 Hz magnetic field on GCSmRNA expression in lymphoma B
cell by mRNA differential display.
Wu RY, Chiang H, Hu GL, Zeng QL, Bao JL
Microwave Lab, Medical College, Zhejiang University, Hangzhou 310031, P.R.
China.
[Record supplied by publisher]
Magnetic fields (MFs) of various characteristics can lead to plethora
effects in biological system. From a molecular point of view, we
hypothesized that there must be a fundamental difference in gene
expression between the MF exposed and the unexposed cell. To identify
the classes of genes that are regulated, 0.8 mT 50 Hz MF-induced changes
in gene expression were examined in a Daudi cell culture using
differential display and reverse transcriptase-polymerase chain reaction.
A candidate cDNA (signatured as MF-CB) that was observed in the
sham-exposed but not in MF-exposed cultures was recovered and reamplified.
After verification by Northern blot, the cDNA was cloned and sequenced.
It was found that 254-base pair of 5'-end MF-CB cDNA clone was identical
to gcs in open reading frame (ORF) range.
Based on the preliminarily sequence, the prolonged length of 5'-end MF-CB
cDNA was obtained by PCR amplification and its sequence analysis showed
the same results as its original fragment. In order to further determine
whether MF-CB cDNA is from gcs, two Northern blots were probed with gcs
and MF-CB cDNA, respectively, and the data revealed signals of the same
size and expression pattern on the two probe filters, which demonstrated
that MF-CB is an EST (expression sequence tag) of gcs. gcs is a gene,
identified recently (GenBank accession number D89866), encoding ceramide
glucosyltransferase (GCS), which has been implicated as a causal element in
human cell growth and differentiation. In an additional experiment,
time-dependent changes in the transcription of gcs induced by 0.8 mT MF were
observed by Northern blot with a sharp and reproducible inhibition effect
after 20 min exposure and a reduction after 20-24 h exposure. The study
demonstrates for the first time that 50 Hz MF can lead to changes in gcs
transcription, which provides a new clue to elucidate the mechanism by which
MF influence cell growth and differentiation. Copyright 2000 Wiley-Liss,
Inc.
PMID: 10972983
Archive provided courtesy of WaveGuide, http://www.wave-guide.org
Reprinted with permission of Roy Beavers, http://www.emfguru.com